CytoCV is a web-based analysis platform for DeltaVision .dv microscopy of mitotic yeast cells. It is designed to keep image files, channel interpretation, scale context, and measurement outputs connected in one browser-based workflow.
The platform supports a modern default analysis path centered on structural segmentation plus red and green fluorescence measurements, while keeping older DAPI-based workflows available as legacy paths when they are needed.
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DIC
Structure
Structural channel used for segmentation.
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mCherry
Red
Red-signal contour and distance context.
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GFP
Green
Green intensity and dot measurements.
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DAPI
Legacy
Legacy nucleus-related analysis path.
Research Need
Why Researchers Need It
Microscopy studies often require researchers to inspect many cells, compare fluorescent signals, and record measurements across repeated experiments. Doing that cell by cell by eye can take time, scale poorly as datasets grow, and introduce inconsistency in how cells are selected or measured.
CytoCV is designed to reduce manual analysis time and help researchers quantify more cells more consistently, while preserving the connection between the original image stack and the numbers produced from it.
Workflow
How the Workflow Works
Researchers upload DeltaVision .dv files, and CytoCV validates the channels required for the selected workflow before analysis proceeds. Preview images are generated first so the file can be checked before segmentation and measurement begin.
The platform then uses DIC, a structural brightfield-like channel, as the input for Mask R-CNN-based cell segmentation. After cells are segmented, CytoCV computes per-cell measurements and presents the results for review and export.
Workflow outputs
- Validation
- Preview images
- Segmentation
- Per-cell measurements
- Review
- Export
Measurements
What CytoCV Measures
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mCherry line intensity
Measures GFP intensity along the line drawn between paired red dot centers.
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GFP dot classification and biorientation
Classifies GFP-dot behavior relative to paired red signals in the modern workflow.
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Green and red contour intensity summaries
Calculates contour-based intensity combinations across red and green channels.
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Nuclear and cellular intensity
Uses either a red-defined or green-defined nucleus contour and measures the opposite channel inside the nucleus and across the whole cell.
DAPI-based measurements remain available as legacy workflows, not the main default path.
Biological Value
Why This Matters Biologically
These measurements help researchers compare signal localization between the nucleus and the whole cell, examine how paired red and green signals relate to one another, and evaluate patterns across yeast populations or mutant conditions.
That makes CytoCV useful for studies where the question depends on where a fluorescent signal is located, how paired markers behave together, or how to compare more cells through a more repeatable analysis process than manual review alone.